2/5/24 Lab Blog

 During the week of 2/5/24 the group and I made 250 mL of multiple types of media for our control (E. coli) and for our samples of D. sonorensis. We made the following types of media: R2A, TSA, NB, TGY, TGY w/ salt (1%, 2%, and 3%), LB, and Mueller Hilton. Our selective and differential media were EMB, PEA, MacConkey, and Mannitol. We also made BHI but did not pour/inoculate with that. We also started the inoculation process for D. sonorensis and actually spread both the control and the samples.

Chad had made the differential media along with the R2A. The recipes for the media we did make are as follows: 

LB - Tryptone 10.0 g/L, Yeast extract 5.0 g/L, NaCl (salt) 10.0 g/L, Agar 15.0 g/L

TGY - Tryptone 3.0 g/L, Glucose 1.0 g/L, Yeast extract 3.0 g/L, Agar 15.0 g/L

TSA - TSA mix 40.0 g/L

NB - NB mix 8.0 g/L, NaCl (salt) 5.0 g/L, Agar 15.0 g/L

TGY 1% salt - Tryptone 3.0 g/L, Glucose 1.0 g/L, Yeast extract 3.0 g/L, Agar 15.0 g/L,

NaCl (salt) 10.0 g/L 

TGY 2% salt - Tryptone 3.0 g/L, Glucose 1.0 g/L, Yeast extract 3.0 g/L, Agar 15.0 g/L, 

NaCl (salt) 20.0 g/L 

TGY 3% salt - Tryptone 3.0 g/L, Glucose 1.0 g/L, Yeast extract 3.0 g/L, Agar 15.0 g/L, 

NaCl (salt) 30.0 g/L 

BHI - BHI mix 37.0 g/L, Agar 15.0 g/L

MHA - MHA Mix 38.0 g/L

The following pictures are the resulting media after being poured.